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guide rna construct pgrna ckb  (Addgene inc)


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    Addgene inc guide rna construct pgrna ckb
    Guide Rna Construct Pgrna Ckb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna+construct+pgrna+ckb/CKB+gRNA+(BRDN0001145509)+(Plasmid+%2376589)/pmc11304550__NIHMS2008921___supplement___Supplemental___2-58-6-24
    Average 90 stars, based on 2 article reviews
    guide rna construct pgrna ckb - by Bioz Stars, 2026-10
    90/100 stars

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    Stable Transfection:

    Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with guide RNA construct pgRNA-CKB with a gRNA cloned into the construct. pgRNA-CKB was a gift from Bruce Conklin (Addgene plasmid # 73501; http://n2t.net/addgene:73501; RRID: Addgene_73501). .. Cancer cell invasiveness was analysed by using a modified Boyden chamber-based assay, CultureCoat 96 Well High BME Cell Invasion Assay (Trevigen, Cat. No: 3483-096-K) or 24-well tanswell inserts with 8μm pores (Sarstedt, Cat no. 83.3932.800) with EHS Matrix Extract as basal 8 membrane (Merck) according to manufacturer’s guidelines.

    Construct:

    Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with guide RNA construct pgRNA-CKB with a gRNA cloned into the construct. pgRNA-CKB was a gift from Bruce Conklin (Addgene plasmid # 73501; http://n2t.net/addgene:73501; RRID: Addgene_73501). .. Cancer cell invasiveness was analysed by using a modified Boyden chamber-based assay, CultureCoat 96 Well High BME Cell Invasion Assay (Trevigen, Cat. No: 3483-096-K) or 24-well tanswell inserts with 8μm pores (Sarstedt, Cat no. 83.3932.800) with EHS Matrix Extract as basal 8 membrane (Merck) according to manufacturer’s guidelines.

    Clone Assay:

    Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with guide RNA construct pgRNA-CKB with a gRNA cloned into the construct. pgRNA-CKB was a gift from Bruce Conklin (Addgene plasmid # 73501; http://n2t.net/addgene:73501; RRID: Addgene_73501). .. Cancer cell invasiveness was analysed by using a modified Boyden chamber-based assay, CultureCoat 96 Well High BME Cell Invasion Assay (Trevigen, Cat. No: 3483-096-K) or 24-well tanswell inserts with 8μm pores (Sarstedt, Cat no. 83.3932.800) with EHS Matrix Extract as basal 8 membrane (Merck) according to manufacturer’s guidelines.

    Plasmid Preparation:

    Article Title: BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma
    Article Snippet: Cells were transfected with Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus. pAAVS1-NDi-CRISPRi (Gen2) was a gift from Bruce Conklin (Addgene plasmid # 73498; http://n2t.net/addgene:73498 ; RRID:Addgene_73498). .. Stable cell lines were differentiated with guide RNA construct pgRNA-CKB with a gRNA cloned into the construct. pgRNA-CKB was a gift from Bruce Conklin (Addgene plasmid # 73501; http://n2t.net/addgene:73501; RRID: Addgene_73501). .. Cancer cell invasiveness was analysed by using a modified Boyden chamber-based assay, CultureCoat 96 Well High BME Cell Invasion Assay (Trevigen, Cat. No: 3483-096-K) or 24-well tanswell inserts with 8μm pores (Sarstedt, Cat no. 83.3932.800) with EHS Matrix Extract as basal 8 membrane (Merck) according to manufacturer’s guidelines.



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    guide rna construct pgrna ckb  (Addgene inc)
    90
    Addgene inc guide rna construct pgrna ckb
    Guide Rna Construct Pgrna Ckb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna+construct+pgrna+ckb/CKB+gRNA+(BRDN0001145509)+(Plasmid+%2376589)/pmc11304550__NIHMS2008921___supplement___Supplemental___2-58-6-24
    Average 90 stars, based on 1 article reviews
    guide rna construct pgrna ckb - by Bioz Stars, 2026-10
    90/100 stars
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